Materials accompanying our paper on neuro-seeER
New paper · August 28, 2026
neuro-seeER distinguishes estrogen-sensitive neurons from neurons actively responding to estrogen. It can be used with standard microscopy and photometry approaches in vitro and in vivo, including longitudinal measurements in behaving animals.
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neuro-seeER plasmids and associated construct information.
Microscopy analysis functions used for Cara, Guthman et al., Neuron 2026.
A permanent, citable snapshot of the analysis code associated with the paper.
AAV delivery, microscopy, snapshot photometry, and recommended validation controls.
The reporter combines stable cre-dependent red fluorescence marking cell identity with a short-lived green signal reporting estrogen-response-element activity. This makes it possible to distinguish cells actively responding to estrogen within a chosen parent population.